rabbit anti phospho ampk substrate motif  (Cell Signaling Technology Inc)

Journal: Frontiers in Pharmacology

Article Title: Ethanol extract of Polygonatum cyrtonema Hua mitigates non-alcoholic steatohepatitis in mice

doi: 10.3389/fphar.2024.1487738

Figure Lengend Snippet: Main experimental primers.

Article Snippet: After blocking with 5% skimmed milk, membranes were incubated with primary antibodies MTTP (Santa, #C2218), SREBP-1c (Abbkin, #ABP53239), PPAR-α (Abbkin, #ABP55667), ApoB (Proteintech, #20578-1-AP), SIRT1 (Abcam, #ab110304), P-AMPK (CST, #5759), AMPK (CST, #2603), P-ACC (CST, #50081), and ACC (CST, #3662) overnight at 4°C.Membranes were incubated with secondary antibody at room temperature for 1 h, followed by washing.

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Ethanol extract of Polygonatum cyrtonema Hua mitigates non-alcoholic steatohepatitis in mice

doi: 10.3389/fphar.2024.1487738

Figure Lengend Snippet: PCE treatment activated the AMPK/SITR1 signalling pathway. (A) Representative Western bolt results of SIRT1, AMPK, ACC, SREBP-1c, PPAR-α, MTTP, and ApoB and quantification results. (B) mRNA relative expression level of SIRT1; (C) mRNA relative expression level of AMPK; (D) mRNA relative expression level of SREBP1; (E) The mRNA relative expression level of PPAR-α; (F) The mRNA relative expression level of MTTP. Data are presented as mean ± SEM (n = 6). * p < 0.05 compared to Control; # p < 0.05 compared to HFD group.

Article Snippet: After blocking with 5% skimmed milk, membranes were incubated with primary antibodies MTTP (Santa, #C2218), SREBP-1c (Abbkin, #ABP53239), PPAR-α (Abbkin, #ABP55667), ApoB (Proteintech, #20578-1-AP), SIRT1 (Abcam, #ab110304), P-AMPK (CST, #5759), AMPK (CST, #2603), P-ACC (CST, #50081), and ACC (CST, #3662) overnight at 4°C.Membranes were incubated with secondary antibody at room temperature for 1 h, followed by washing.

Techniques: Western Blot, Expressing, Control